BFA-Independent PIN Targeting by Phosphorylation-Based Sequence Modification.
(A) to (F) Confocal live cell imaging of PIN1-GFP variants. Bars = 10 μm.
(A) and (B) PIN1-GFP localization in the root (untreated in [A]) is sensitive to BFA treatment, leading to PIN1-GFP accumulation in BFA compartments (B).
(C) and (D) Nonphosphorylated PIN (PIN1S123A-GFP) targeting in untreated (C) and BFA-treated seedlings (D).
(E) and (F) Phosphorylation-mimicking PIN1S123E-GFP localization in untreated (E) and BFA-treated seedlings (F), indicating that the phosphorylated PIN proteins preferentially traffic in a BFA-resistant manner.