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. 2009 Dec;21(12):3839–3849. doi: 10.1105/tpc.109.071639

Figure 7.

Figure 7.

BFA-Independent PIN Targeting by Phosphorylation-Based Sequence Modification.

(A) to (F) Confocal live cell imaging of PIN1-GFP variants. Bars = 10 μm.

(A) and (B) PIN1-GFP localization in the root (untreated in [A]) is sensitive to BFA treatment, leading to PIN1-GFP accumulation in BFA compartments (B).

(C) and (D) Nonphosphorylated PIN (PIN1S123A-GFP) targeting in untreated (C) and BFA-treated seedlings (D).

(E) and (F) Phosphorylation-mimicking PIN1S123E-GFP localization in untreated (E) and BFA-treated seedlings (F), indicating that the phosphorylated PIN proteins preferentially traffic in a BFA-resistant manner.