Skip to main content
. Author manuscript; available in PMC: 2010 Sep 15.
Published in final edited form as: J Immunol. 2009 Aug 26;183(6):3598. doi: 10.4049/jimmunol.0901244

FIGURE 2.

FIGURE 2

Effect of wild-type hIL-15 and muteins on 32Dβ cell proliferation. 32Dβ cells (hIL-15Rβ-mγ-positive) were incubated with increasing concentrations of hIL-15-containing proteins for 48 h and proliferation assays were performed as described in Figure 1. A, Dose-responses curves of 32Dβ cell proliferation assessed with different concentrations of c264scTCR/hIL-15 wild type (■) and c264scTCR/hIL-15N72D (□) fusions. B, Proliferation assays of 32Dβ cells using c264scTCR/hIL-15 and nine hIL-15 mutein fusion proteins were carried out. The activity based on EC50 of c264scTCR/hIL15 wild-type fusion was normalized to 100% and the relative activities of mutein fusions were determined. C, Dose-responses curves of 32Dβ cell proliferation assessed with different concentrations of rhIL-15 wild type (■) and rhIL-15N72D (□) proteins. The results are representative of at least three independent experiments.