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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: J Mol Biol. 2009 Dec 4;395(5):1007. doi: 10.1016/j.jmb.2009.11.071

Table 2.

Steady-state kinetic parameters for dCTP incorporation by Dpo4 T239Wa

Template base kcat
s-1 		Km,dCTP
μM		 kcat/Km,dCTP
mM-1 s-1 		kcat/Km,dCTP
relative to G
G 0.057 ± 0.001 5.9 ± 0.5 9.7 1.0
N2-MeG 0.078 ± 0.003 9.7 ± 1.8 8.0 0.82
N2-IbG 0.011 ± 0.001 105 ± 30 0.10 0.010
N2-BzG 0.0061 ± 0.0003 36 ± 6 0.17 0.018
N2-NaphG 0.015 ± 0.001 124 ± 16 0.12 0.012
N2-AnthG 0.0031 ± 0.001 5.1 ± 0.6 0.61 0.062
N2,N2-Me2G (11 ± 0.1) × 10-6 190 ± 44 5.8 × 10-5 5.9 × 10-6
a

The extent of conversion of primer to the product was kept < 20% by adjustment of the enzyme concentration and reaction time.