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Pre-steady-state kinetic analysis of dCTP incorporation by Dpo4 T239W
| Template base |
kpol s-1 |
Kd,dCTP μM |
kpol/Kd,dCTP mM-1 s-1 |
kpol/Kd,dCTP relative to G |
|---|---|---|---|---|
| G | 1.1 ± 0.1 | 35 ± 6 | 31 | 1.0 |
| N2-MeG | 2.8 ± 0.2 | 99 ± 23 | 28 | 0.90 |
| N2-IbG | 0.20 ± 0.01 | 89 ± 16 | 2.2 | 0.071 |
| N2-BzG | 0.48 ± 0.02 | 330 ± 40 | 1.5 | 0.15 |
| N2-NaphG | 0.26 ± 0.01 | 530 ± 60 | 0.49 | 0.016 |
| N2-AnthG | 0.70 ± 0.03 | 120 ± 16 | 5.8 | 0.19 |
