Table 2.
Summary results of flow cytometric analyses in Cardamine species, using DAPI (relative DNA content, given in arbitrary units) and PI (absolute DNA content, given in pg) fluorochromes
| (a) Relative DNA content (a.u.) | ||||||
|---|---|---|---|---|---|---|
| Taxon, DNA ploidy level | DNA content: min.–max. (mean) | Intracytotype variation, max./min. (%) | Mean relative DNA content per monoploid genome | CV of samples, min.–max. (mean) (%) | CV of standard, min.–max. (mean) (%) | No. of plants |
| torrentis s.l.: | ||||||
| valida 2n ≈ 4x | 0·651–0·737 (0·687) | 13·21 | 0·172a | 1·83–4·43 (2·582) | 1·52–3·5 (1·994) | 21 |
| torrentis s.s. 2n ≈ 8x | 1·105–1·189 (1·138) | 7·6 | 0·142 | 1·33–3·07 (1·756) | 1·29–4·47 (1·964) | 35 |
| amaraeiformis 2n ≈ 4x | 0·663–0·763 (0·696) | 15·08 | 0·174a | 2·06–4·36 (2·896) | 1·9–3·43 (2·286) | 10 |
| yezoensis 2n ≈ 6x | 0·674–0·785 (0·715) | 16·47 | 0·119c | 1·69–5·37 (2·418) | 1·27–3·12 (1·945) | 60 |
| yezoensis 2n ≈ 6x (excl. JP 135, JP 136) | 0·674–0·759 (0·712) | 12·61 | 0·119c | 1·69–5·37 (2·411) | 1·27–3·12 (1·927) | 57 |
| yezoensis 2n ≈ 8x | 0·842–0·852 (0·847) | 1·19 | 0·106b | 2·41–3·18 (2·708) | 2·12–2·98 (2·485) | 2 |
| yezoensis 2n ≈ 10x | 0·995–1·073 (1·027) | 7·79 | 0·103b | 1·44–3·25 (2·007) | 1·58–3·57 (2·364) | 16 |
| yezoensis 2n ≈ 11x | 1·082–1·137 (1·110) | 5·08 | 0·101b | 1·65–2·6 (2·033) | 1·68–2·71 (2·1) | 5 |
| yezoensis 2n ≈ 12x | – | – | – | – | – | – |
| schinziana 2n ≈ 6x | 0·682–0·755 (0·713) | 10·7 | 0·119c | 1·56–4·13 (2·422) | 1·56–3 (1·963) | 12 |
| schinziana 2n ≈ 8x | 1·014–1·054 (1·031) | 3·88 | 0·129 | 1·58–2·3 (2·011) | 2·11–2·95 (2·61) | 3 |
| (b) Absolute DNA content (pg) | ||||||
| Taxon, DNA ploidy level | DNA content, min.–max. (mean) | Intracytotype variation, max./min. (%) | Mean monoploid genome size | CV of samples, min.–max. (mean) (%) | CV of standard, min.–max. (mean) (%) | No. of plants |
| torrentis s.l.: | ||||||
| valida 2n ≈ 4x | 1·419–1·587 (1·493) | 11·84 | 0·373a | 1·76–9·35 (5·971) | 1·32–5·69 (4·29) | 18 |
| torrentis s.s. 2n ≈ 8x | 2·26–2·469 (2·362) | 9·25 | 0·295 | 2·02–6·19 (4·411) | 1·3–7·03 (4·255) | 33 |
| amaraeiformis 2n ≈ 4x | 1·411–1·64 (1·495) | 16·23 | 0·374a | 1·42–5·36 (2·583) | 1·19–2·85 (1·716) | 10 |
| yezoensis 2n ≈ 6x | 1·394–1·66 (1·482) | 19·08 | 0·247c | 1·86–8·96 (5) | 1·35–7·98 (3·656) | 57 |
| yezoensis 2n ≈ 6x (excl. JP 135, JP 136) | 1·394–1·583 (1·471) | 13·56 | 0·245c | 1·99–8·96 (5·21) | 1·35–7·98 (3·747) | 52 |
| yezoensis 2n ≈ 8x | 1·736–1·786 (1·756) | 2·88 | 0·220b | 2·5–6·12 (3·389) | 1·18–3·11 (2·011) | 3 |
| yezoensis 2n ≈ 10x | 1·97–2·099 (2·051) | 6·55 | 0·205b | 4·4–7·57 (5·6) | 3·19–5·78 (3·808) | 10 |
| yezoensis 2n ≈ 11x | 2·248–2·362 (2·308) | 5·07 | 0·210b | 2·08–5·25 (3·641) | 1·39–4·18 (2·803) | 6 |
| yezoensis 2n ≈ 12x | 2·47–2·528 (2·499) | 2·35 | 0·208b | 1·96–4·57 (3·015) | 1·4–2·66 (1·74) | 2 |
| schinziana 2n ≈ 6x | 1·363–1·565 (1·448) | 14·82 | 0·241c | 1·86–9·79 (4·915) | 1·4–5·61 (3·557) | 14 |
| schinziana 2n ≈ 8x | 2·008–2·081 (2·045) | 3·64 | 0·256c | 4·96–6·48 (5·682) | 3·04–5·41 (4·047) | 2 |
Data are summarized for each ploidy level of a given taxon. For hexaploid C. yezoensis, data are given also without JP 135 and JP 136, which most likely also comprise aneuploids. Means of relative DNA content and monoploid genome size are significantly different at P < 0·05 (Tukey–Kramer test), unless marked by the same lower-case letter.