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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: J Sex Med. 2009 Jun 9;6(9):2480. doi: 10.1111/j.1743-6109.2009.01349.x

Figure 4.

Figure 4

Quantification of Hip, Shh, and Ptch1 RNA and hedgehog-interacting protein (HIP) in cavernous nerve (CN)-cut rats. (A) Quantification of SHH, PTCH1, and HIP proteins in control, 2 and 5 days CN-injured Sprague-Dawley rat penises. SHH and PTCH1 proteins were significantly decreased at 2 and 5 days after CN injury. HIP was unchanged at 2 days after CN injury but was significantly decreased 1.4-fold 5 days after CN injury. (B) Hip RNA was quantified by real-time reverse transcriptase-polymerase chain reaction (RT-PCR) using Rpl19 as an internal standard. There was no change in Hip expression at 2 days after CN injury however Hip expression was significantly increased twofold at 5 days after CN injury (P value = 0.03). (C) Shh RNA was quantified by real-time RT-PCR using Rpl19 as an internal standard. Shh expression significantly increased 2.4 and 1.7-fold at 2 and 5 days after CN injury (P values = 0.001 and 0.002). (D) Ptch1 RNA was quantified by real-time RT-PCR using Rpl19 as an internal standard. Ptch1 expression significantly increased 4.6- and 2.3-fold at 2 and 5 days after CN injury (P values = 0.05 and 0.03).