Figure 10.

MKLP2 localization is altered in Mob1A/Mob1B-depleted cells. (A) Thymidine-synchronized RPE1 cells were transfected with either nontargeting control (left) or Mob1A/Mob1B siRNA's (right), and probed for INCENP and MKLP2 localization. In contrast to controls (a–c), MKLP2 recruitment during early anaphase was disrupted in Mob1A/B-depleted cells (d–f), but by mid-late anaphase (j–l), MKLP2 recruitment to the midzone resembled controls (g–i). Bar, 10 μm. (B) Disruption of MKLP2 localization to the central spindle occurred in 63.7% of cell depleted of Mob1A/1B during early anaphase (n = 92 cells, SE = 0.0358), but by mid-late anaphase, MKLP2 localization matched controls (n = 102).