Figure 2.

GFP-Mob1 localization in dividing HeLa cells. Optical sections of HeLa cells transfected with GFP-Mob1A revealed that Mob1 recruitment to centrosomes began in late G2 phase and was maintained through cytokinesis (A–F). Kinetochore localization was most prominent during prometaphase (B) but lost during anaphase and cytokinesis (D and E). Mob1 localized to the spindle midzone during anaphase and was enriched in the midbody late in cytokinesis (D and E). Treatment with 250 nM nocodazole failed to disrupt either spindle pole- or kinetochore localization (F). Bar, 10 μm.