Figure 4.

Plk1 is required for Mob1 recruitment to the spindle poles. GFP-Mob1A–expressing HeLa cells were transfected with nontargeting control and Plk1 siRNA (for Western blot confirmation, see Supplemental Figure 4). Twenty-four hours post-siRNA transfection, cells were processed for localization of γ tubulin (A–F) and Plk1 (G–L). In the absence of Plk1, cells formed a monopolar spindle, and although Mob1 was lost from the centrally located spindle pole (D–F), kinetochore localization was unaffected (D–F, J–L, and M–O). Bar, 10 μm.