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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Plant J. 2008 Dec 16;58(2):275–286. doi: 10.1111/j.1365-313X.2008.03778.x

Figure 7. AtMYB30 interact with BES1 in vitro and in vivo.

Figure 7

(a-b) Co-expression of BES1-EYFP-C and EYFP-N-AtMYB30 lead to the reconstitution of EYFP activity as observed under an OLYMPUS IX71 fluorescence microscope with a YFP filter. The protoplasts with positive signals in the nuclei are indicated by arrows.

(c) Co-expression of BES1-YFP-C plus YFP-N did not produce any positive signal.

(d) BES1 interacts with AtMYB30 by GST pull-down assay. Full-length BES1 (aa 1-335), including DNA BD (DNA binding domain), Phospho (BIN2 phosphorylation domain), PEST motif, and the C-terminus (C), as well as various deletions are shown. Approximately equal amounts of GST, GST-AtMYB30 and MBP-BES1 (top gel) or GST, GST-BES1 deletions and MBP-AtMYB30 (middle gel) proteins were used in the assays as shown by Comassie stained gel (bottom gel). The proteins were detected by Western blotting with anti-MBP antibody (NEB).

(e) Models AtMYB30 function in BES1-regulated gene expression. BES1 activates the expression of AtMYB30, which bind to the target gene promoters together with BES1 to synergistically activate a subset of BR target genes. Other BES1 induced transcription factors (TFs) may function similarly as AtMYB30 to regulate other BR-target genes.