Figure 6.

Identification of IC GAD67 neurons that project to the MGB. a, One neuron with VGLUT2⁺ axosomatic endings (white arrow). b, Large GAD67⁺ with axosomatic endings (white arrow) and a second GAD67⁺ neuron (black arrow) without axosomatic endings. c, After an FG injection into the medial geniculate body, retrogradely labeled FG-positive cells were found in the IC (white arrows and arrowheads). d, The large GAD67⁺ cells with VGLUT2⁺ axosomatic endings were labeled with FG (white arrow). GAD67⁺ neurons without VGLUT2⁺ axosomatic endings were not labeled with FG (black arrows). The majority of FG-positive cells are negative for GAD67 (white arrowheads). Green, VGLUT2; red, GAD67; blue, FG. Scale bar: (in d) a–d, 50 μm.