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Additional PPARγ agonists enhance iTreg conversion. CD4+CD25– C57BL/6 splenic Teffs (1×105) were cultured with 1 × 104 CD11c+ Bm12 splenic DCs in the presence of TGF-β and VC or 40 μM Rosi (A) or VC or 15d-PGJ2 (15d; B). Some wells contained 1 μM or 5 μM GW9662. The cells were stained for CD4 and Foxp3 expression on Day 5. Results are shown gated on CD4+ cells.
