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. 2010 Feb 2;5(2):e8897. doi: 10.1371/journal.pone.0008897

Figure 7. P2Y11 is required for frequent calcium elevation and coordinated cell polarization.

Figure 7

(A) The effect of P2Y11-MO on the frequency of calcium flashes in chordamesodermal tissues near the boundary with ectoderm explants. (uninj.: morpholino-uninjected, cont: control morpholino, MO: morpholino) (B) The cell alignment in the P2Y11-MO-injected chordamesodermal tissue (RFP-membrane (red)) near the boundary with ectoderm (GFP-membrane (green)). The several rounded cells were contained (arrowheads) and the perpendicular alignment was relatively disturbed. (C) The effect of P2Y11-MO on the frequency of calcium flashes in the chordamesodermal tissues at the area of crawling cells under the furrow of silicone block. (C′) The ratio of cell alignment in (C), and coordinated cell alignment was not established in the MO-injected explants under the silicone furrow. (D, D′) cell alignment after CE in wild type DMZ (D), and morpholino-injected DMZ (D′). The cells showed coordinated alignment with proper polarity in D while their polarity was disrupted in D′. The lower rose diagrams show the angles of long axis of each cells in (D) and (D′). (E) The ratio of coordinated cell alignment in wild type DMZ and P2Y11-MO-injected DMZ. In the morpholino-injected DMZ showed random cell alignment compared with wild type. (E′) The ratio of boundary formation in wild type DMZ and P2Y11-MO-injected DMZ. Most of the morpholino-injected explants did not form clear boundary. (F) The number of calcium flashes in wild type DMZ and P2Y11-MO-injected DMZ.