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. 2010 Feb 2;5(2):e9020. doi: 10.1371/journal.pone.0009020

Figure 3. HCF-1 Basic region duplications can rescue tsBN67 colony forming activity at the nonpermissive temperature.

Figure 3

A) Schematic of the HCF-1 Basic region duplication mutants. Each domain, labeled 1 and 2, has been selectively deleted, duplicated or placed in inverted position. B) Western blot analysis of the expression of HCF-1 Basic region mutants in 293 cells. Red asterisks indicate the expected proteins based on their predicted size. C) tsBN67 colony formation assays. Each clone has been tested at permissive and nonpermissive temperature in triplicate as shown in columns A, B, and C and plated in duplicate. D) Spectrophotometric quantitation of the ability of deletion mutants to rescue cell proliferation at the nonpermissive temperature. The error bars are standard error of the mean. E) Colony morphology of selected clones at nonpermissive temperature.