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. 2010 Feb 2;5(2):e9020. doi: 10.1371/journal.pone.0009020

Figure 4. HCF-1 Basic region Domains 1 and 2 can individually promote HeLa cell G1-to-S phase progression.

Figure 4

HeLa cells that stably express different HCF-1 silencing resistance mutants were tested for their ability to bypass G1-to-S arrest induced by silencing of endogenous HCF-1 with RNAi. A) Immunofluorescence analysis of HeLa cells silenced with Luciferase RNAi oligos and with HCF-1 siRNA oligos. Three panels are showed for each clone tested. The left panel shows DNA staining with DAPI. The middle panel shows cells probed with antibody for BrdU incorporation. The right panel shows the level of endogenous HCF-1 remaining after siRNA treatment as detected by the HCF-1C antibody (αH12). B) Percentage of BrdU positive cells after 72 hours of siRNA silencing as determined by the immunofluorescence results normalized to HCF-1N1011. Sample sizes were as follows: HeLa, n = 1127; HCF-1N1011-ΔBasic, n = 881; HCF-1N1011-D1, n = 1920; HCF-1N1011-D11, n = 859; HCF-1N1011-D2, n = 705; HCF-1N1011-D22, n = 1798; HCF-1N1011-D21, n = 1478; and HCF-1N1011, n = 1475. The error bars are standard error of the mean. *p<0.05 (Mann-Whitney U test), comparing the HCF-1N mutants to HCF-1N1011-ΔBasic.