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αFLAG immunoprecipitates of the stable HeLa cell lines with the single, duplicated, and swapped Basic region Domains 1 and 2 were analyzed by immunoblot with an αSin3a antisera. A) Recovery of the FLAG tagged HCF-1 recombinant proteins was verified by immunoblot analysis with the αFLAG antibody. B) Sin3a in the supernatant (bottom) and immunoprecipitates (top) was determined by αSin3a immunoblotting.
