Table 1.
Determination of binding affinities for different M10-OL1 and M10-O1 complexes by ITC (see SI Methods). The acronym Bel M10 indicates the M10 I55N variant of the Belgian mutation. The parameters N, KA, ΔH, T, ΔS, ΔG indicate binding stoichiometry, binding affinity, binding enthalphy, temperature, binding enthropy, and Gibbs’ free energy of binding, respectively
| Interaction | N | KA (105 M-1) | ΔH (kcal/mol) | TΔS (kcal/mol) | ΔG (kcal/mol) |
| M10-OL1 | 1.04 ± 0.008 | 12.6 ± 1.5 | 3.87 ± 0.34 | 12.0 | -8.1 |
| M10-O1 | 1.05 ± 0.005 | 3.7 ± 0.2 | 11.16 ± 0.68 | 18.6 | -7.4 |
| M10-OL1 F17R | 0.99 ± 0.014 | 7.8 ± 1.3 | 0.66 ± 0.11 | 8.6 | -7.9 |
| M10-O1 R15F | 1.04 ± 0.003 | 24.2 ± 1.4 | 5.64 ± 0.18 | 14.2 | -8.6 |
| Bel M10-OL1 | 1.05 ± 0.005 | 11.7 ± 0.9 | 3.66 ± 0.20 | 11.8 | -8.1 |
| Bel M10-O1 | 1.07 ± 0.010 | 6.4 ± 0.7 | 9.6 ± 0.98 | 17.3 | -7.7 |