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. Author manuscript; available in PMC: 2011 Feb 3.
Published in final edited form as: Vaccine. 2009 Nov 26;28(5):1300. doi: 10.1016/j.vaccine.2009.11.020

Figure 6. Gag-specific IgG response: Suppression with agonistic anti- 4-1BB Ab, while preservation/augmentation with membrane bound 4-1BBL DNA expressed in cis.

Figure 6

A, BALB/c mice (n=3) were primed with 100μg of HIV-1 Gag DNA vaccine with or without the agonistic anti-4-1BB Ab at week 0 and boosted with MVA-Gag at week 12. Sera were collected at 1 week after the boost and total Gag-specific IgG was determined by ELISA. B, Mice (n=10) were primed with plasmid DNA (20μg) at week 0 and boosted with MVA-Gag at week 4. Gag-specific IgG was determined by ELISA at one week following the boost. C, Mice (n=10) were primed and boosted with plasmid DNA (20μg) at week 0 and at week 4 respectively. Total Gag-specific IgG was evaluated at two weeks after the DNA boost. Sera from unimmunized naïve mice were used as negative control. The bars represent mean ± SEM of Gag-specific IgG titer in serum. *, p= 0.05. NA, Not available.