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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: J Reprod Immunol. 2009 Dec 5;84(1):16. doi: 10.1016/j.jri.2009.09.009

Figure 6. TWEAK potentiates the effects of Pam3CysSK4 and Neisseria gonorrhoeae on HEK293/TLR2 cell activation.

Figure 6

Figure 6

HEK293/TLR2 cells were stimulated with increasing concentrations of Pam3CysSK4 (5, 50, 500 ng/ml; P3CSK4) or N. gonorrhoeae (multiplicity of infection = 0.1, 1, 10 CFU; GC), in the presence or absence of 1 μg TWEAK per ml. After 24 hours, culture supernatants were harvested and assayed for IL-8 by ELISA. The values are derived from IL-8 standard curves and represent the mean ± the SEM from triplicate wells. Significance is noted as follows for P3CSK4 or GC alone vs. TWEAK + P3CSK4 or GC: *, p<0.001; **, p<0.0001. For unstimulated vs. TWEAK alone, p<0.001. These results are representative of two independent experiments.