Figure 6. Differential expression validated by reverse transcripton quantitative PCR.
RT-qPCR was applied as independent means to validate differential expression as detected by the microarray approach. Primers were designed for selected features and qPCR was performed with the same templates that were used to generate the cDNA probes for the microarray experiments. While the results tended to indicate greater fold change in expression, RT-qPCR results did correlate closely to the trends observed in transcript expression for all the array treatments.