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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Peptides. 2009 Oct 29;31(1):9. doi: 10.1016/j.peptides.2009.10.004

Fig. 6. Bactericidal activity of truncated prosegment variants is fully inhibited.

Fig. 6

Exponentially-growing bacteria (~ 5 × 106 CFU/mL) Vibrio cholerae (A), Escherichia coli (B), Salmonella Typhimurium CS022 (C), Staphylococcus aureus (D), Salmonella Typhimurium 14028S (E), and Listeria monocytogenes (F) were exposed to peptides at 37 °C in 50 μl 10 mM PIPES buffer supplemented with 1% TSB for 1 h. Assays were performed in triplicate, and error bars denote standard deviation. Following peptide exposure, the bacteria were plated on TSB-agar, incubated overnight at 37 °C (see Materials and Methods, Section 2.4) [17], and surviving bacteria were counted as CFU at each peptide concentration. Values at or below 1 × 103 CFU/ml signify absence of colonies after overnight growth, the detection limit of the assay. Symbols: Crp4 (-●-), proCrp4(20–92) (-○-), (Δ44–53)-proCrp4(-▼-), (Δ44–58)-proCrp4 (-▽-). For all species tested, exposure to native proCrp4 and truncated variants has no effect on bacterial cell survival.