A–C. The effect of amfenac, celecoxib, and SC-560 on VEGF-induced HRMEC tube formation. (A) HRMEC tube formation was induced by 25 ng/ml VEGF, and this induction was significantly inhibited by amfenac (* p ≤ 0.001), the COX-2-selective NSAID celecoxib (* p ≤ 0.001; † p ≤ 0.006), and, at higher concentrations, the COX-1 selective NSAID SC-560 (* p ≤ 0.001; ‡ p ≤ 0.01). Each bar represents the mean ± SD. (B) A representative image of tube formation in VEGF-stimulated HRMEC. (C) A representative image of tube formation in VEGF-stimulated HRMEC treated with 1 μM amfenac. Amfenac-treated HRMEC demonstrate reduced tube formation.