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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: Cell Signal. 2009 Nov 20;22(4):584. doi: 10.1016/j.cellsig.2009.11.010

Figure 4. Alteration on Akt target and Bcl-2 family proteins.

Figure 4

A. Phosphorylated and total p70S6K were detected using their specific antibodies using immuoblotting. Increased Akt activity in Ad.AC6 infected cells was associated with increased phosphorylation of p70S6K protein. In contrast, ß1AR expression was associated with decreased phosphorylation of p70S6K protein.

B. Phosphorylated GSK3 α and β subunits and total GSK3 β protein were detected using their specific antibodies. Increased AC6 was associated with 5-fold increase in phosphorylation of GSK3 α and β subunits and ß1AR expression was associated with 2.5-fold increase in phosphorylation of these proteins.

C. Bcl-2 and phospho136 Bad proteins were detected using their specific antibodies. Increased AC6, but not ß1AR, was associated with a 2-fold increase in Bcl-2 expression.

D. Bax expression was detected using anti-Bax antibody and compared with Bcl-2 protein. Increased ß1AR protein was associated with an increased ratio of Bax/Bcl-2. Bars in graphs denote mean±SD derived from three independent experiments.