Fig. 4.

Both GT and GHT fusion proteins showed intrinsic in vivo bioactivity after subcutaneous dosing. The hypophysectomized rats that did not gain body weight during the pre-screening period were randomized to four groups and evaluated for intrinsic bioactivity. The fasted rats were then subcutaneously administered (s.c.) with vehicle control, hGH (0.25 mg/kg), GT (1.25 mg/kg), or GHT (1.25 mg/kg), for 7-consecutive days, respectively. The weight gain was determined by subtracting the weight on the day of dosing (day 1) from that of last day of dosing (day 8). Data represent mean of body weight gain ± SEM (n = 5, except GHT s.c. where n = 4).