Skip to main content
. Author manuscript; available in PMC: 2010 Feb 2.
Published in final edited form as: Leuk Res. 2009 Jun 7;34(2):190. doi: 10.1016/j.leukres.2009.05.007

Table 3.

t(14;18)-positive and t(14 ;18)-negative cases based on FISH versus t(14;18)-PCR assays run on archival paraffin-embedded lymphoma samples

t(14;18)-FISH t(14;18)-PCR
Positive Negative Unreadableb Total
Positive 40 32 10 82
Negative 7 53 10 70
Unreadablea 13 25 37 75
Total 60 110 57 227

t(14;18) status was determined using Vysis LSI IGH/BCL2 Dual Color, Dual Fusion FISH assays. PCR assays used a Jh consensus primer, an MBR1 primer corresponding to the BCL2 major breakpoint region (MBR), and a second primer 360 base pairs upstream of MBR1. Assays were run on archival paraffin-embedded tumor sections.

a

Unreadable FISH assays could not be scored because of inadequate sample or FISH signal strength.

b

Unreadable PCR assays were negative for the human hemoglobin beta (HBB) internal control and t(14;18).