Chronic administration of a Cox-2 inhibitor reduces the number of VV-specific antibody secreting cells. C57BL/6 mice were administered either vehicle or SC-58125 (5 mg/kg) starting seven days before infection with VV (1×106 PFU) and continuing on to day 27. (A) Bone marrow cells and splenocytes, harvested on day 28, were assessed by ELISPOT for the preence of VV-specific IgM secreting cells. Splenocytes were further evaluated by ELISPOT for IgG1, Ig2a (B), IgG2b or IgG3 (C) isotype secreting cells. Numbers of spots per 1 × 106 cells are shown. (D) Average spot size is depicted. Data are represented as mean ± SEM, * p <0.05, **p = 0.07.