Figure 3. Stx1 interactions with T84 cells cause mistargeting of apical membrane proteins DPPIV, villin and NHE2 into the basolateral compartments through the loss of intracellular galectin-3.
Single XY projections from 3D reconstruction and optical XZ projections of intracellular distribution of protein of interest (in green by immunostaining with corresponding primary antibody and Alexa Fluor 488 secondary antibody). Cell nuclei (blue) labeled by Hoechst.