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Detection of early complement factors of the alternative pathway and IgG natural antibody. The nIgM-ischemic Ag complexes were captured in antibody-coated microtiter wells as described in Figure 1. a) Detection of C3 in the captured immunocomplexes from intestinal tissues. b) Detection of Factor P. c) Detection of IgG. Asterisks indicate statistical significance (Student's t test; * p<0.05). Error bars indicate SEM.
