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. Author manuscript; available in PMC: 2011 Feb 17.
Published in final edited form as: Neuroscience. 2009 Dec 3;165(4):1390. doi: 10.1016/j.neuroscience.2009.11.071

Figure 1. 5-HT reduced the amplitude of eEPSCs in the BNST.

Figure 1

Whole cell patch clamp recordings were made on BNST slices and EPSCs were evoked by stimulating the stria terminalis. Membrane potential was held at −60 mV. A) In one BNSTAL neuron, application of RS-CPP (10 μM) had no noticeable effect on the eEPSCs, whereas DNQX (20 μM) nearly completely blocked eEPSCs. Notice that the eEPSCs traces in the ACSF and RS-CPP were overlapped. B) In another BNSTAL neuron, RS-CPP application caused a leftward shift of the decay phase of eEPSCs, whereas did not affect the rising phase and peak amplitude of eEPSCs. Subsequently application of DNQX blocked the residual component of eEPSCs. Inset: the NMDA receptor-mediated EPSCs was revealed by subtracting the eEPSCs traces obtained before and during RS-CPP application. C) Representative traces showing the eEPSCs recordings before, during and after 5-HT application. 5-HT suppressed the amplitude of eEPSCs, an effect that reversed after 10 min of wash with ACSF. D) The time course of 5-HT (50 μM) action on eEPSCs amplitude indicated a fast activating and recovery pattern. **, p<0.01 vs baseline. E) Dose-response curve of the inhibiting effect of 5-HT on eEPSCs in BNST, the IC50 was 4.0 μM. The numbers of neurons tested in each 5-HT concentration were between 6 and 10.