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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: Mol Cell Endocrinol. 2009 Aug 22;315(1-2):159. doi: 10.1016/j.mce.2009.08.011

Figure 2. Glucocorticoid receptor binds to the PDK4 promoter.

Figure 2

Chromatin immunoprecipitation (ChIP) assays were conducted with the PDK4 gene and antibodies to the glucocorticoid receptor (GR). Primary rat hepatocytes were treated with dexamethasone (100 nM) or dexamethasone plus insulin (100 nM). After 4 hours of hormone treatment, the cells were crosslinked with 1% formaldehyde and the ChIP assays conducted as described in the materials and methods. A. The location of the primer sets used for PCR amplification is indicated in the model. B. Representative PCR products are shown for each primer set. Also shown are the PCR amplifications for input DNA and the IgG control. C. The PCR products were quantified with Quantity One software. The changes in receptor binding are shown. The ChIP assays were repeated four times on independent preparations of primary rat hepatocytes. The p< 0.01 for the insulin inhibition is indicated by the * asterisk and p< 0.05 indicated by #.