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. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: Mol Cell Endocrinol. 2009 Aug 22;315(1-2):159. doi: 10.1016/j.mce.2009.08.011

Figure 5. ERRα participates in regulation of PDK4 expression.

Figure 5

A. At the top, models of the −6529/+78 PDK4-luciferase vectors are shown. McA-RH7777 cells were transfected with PDK4- luciferase vectors and 100 nM dexamethasone or 100 nM insulin were added for 24 hours. The luciferase activity was corrected for renilla and protein content. Transfections were repeated four to six times in duplicate. B. McA-Rh7777 cells were infected with adenoviruses expressing shRNA for ERRα (Ad-shERRα) or the (Ad-NC). The infection conditions are described in the Materials and Methods. RNA was isolated, and the mRNA abundance of PDK4 and ERRα was measured by real time PCR. The data are expressed as the mean of the -fold induction ± S.E. of mRNA abundance of the shRNA ERRα infected cells relative to the NC-infected cells. The data represent the average of four independent experiments.