Acetylation of histones H2B, H3, and H4 was assessed by western blotting using specific antibodies. The results were expressed as ratios to beta-actin. RGC-32 silencing induced an increase in the expression of histones H2BK5ac, H2BK15ac, H3K9ac, H3K18ac, and H4K8ac. Data are mean ± SEM from three separate experiments.