Skip to main content
. Author manuscript; available in PMC: 2011 Feb 1.
Published in final edited form as: Exp Mol Pathol. 2009 Oct 31;88(1):67. doi: 10.1016/j.yexmp.2009.10.010

Figure 9. Effect of RGC-32 knockdown on cell cycle phases.

Figure 9

After an 18-h stimulation with RPMI containing 10% FBS, BrdU was incorporated into SW480 cells transfected with CTR shRNA or RGC-32 shRNA. DNA synthesis was assessed by BrdU incorporation, and DNA content was determined using 7-aminoactinomycin D and flow cytometry. Results are means ± SEM from three separate experiments. RGC-32 knockdown induced S-phase and G2/M-phase entry, with 3.4% more cells being found in S phase and 3.2% more cells in G2/M phase in the SW480 RGC-32-transfected cells than in the shCTR-transfected cells. Data are means ± SEM from three independently conducted experiments.