Table 1.

Primers and hybridization probes used for Real-Time PCR.

Gene symbol	Primers sequence	Product (bp)
CDH1	For: 5′-CAATCTCAAGCTCATGGATAACC-3′	187
	Rev: 5′-GAGCAGCAGAATCAGAATTAGC-3′
HIST1H2BD	For: 5′-ACTTTGCCAAGGGAGAGACA-3′	104
	Rev: 5′-TTTCAGGCAGATGAGACTTCC-3′
p21	For: 5′-GAAGACCATGTGGTCCTGTCAC -3′	172
	Rev: 5′-AGGGCTTCCTCTTGGAGAAGAT -3′
p53	For: 5′-GTCCCAAGCAATGGATGATT-3′	104
	Rev: 5′-GAGCAGCCTCTGGCATTCT-3′
RGC-32	For: 5′-AGGAACAGCTTCAGCTTCAGT-3′	152
	Rev: 5′-GCTAAAGTTTTGTCAAGATCAGCA-3′
WEE1	For: 5′-TGCTGGTGCTGAACCTCT-3′	282
	Rev: 5′-GCCATCTGTGCTTTCTTGAG-3′
	Hyb 1:GCAACTCTGTAAATTCTTGGGAAAGCAC-FL
	Hyb 2:LC640-GTGGTATCCGAGGTAATCTACCCTGTCTG-PH
L13	For: 5′-CGTGCGTCTGAAGCCTACA-3′	227
	Rev: 5′-GGAGTCCGTGGGTCTTGAG-3′
β-catenin	For: 5′-ACAGGAAGGGATGGAAGGT -3′	171
	Rev: 5′-ACAGTACGCACAAGAGCCTC -3′
18S	For: 5′-GTAACCCGTTGAACCCCATT-3′	151
	Rev: 5′-CCATCCAATCGGTAGTAGCG-3′

For, forward primer; Rev, reverse primer; Hyb 1, Hyb 2, hybridization probes; Bp, base pairs; CDH1, Cadherin 1, type 1, E-cadherin; HIST1H2BD, H2B histone family, member B; p53, tumor protein p53; p21, cyclin-dependent kinase inhibitor 1A; RGC-32, response gene to complement 32; WEE1, WEE1 homolog; 18S, 18S ribosomal RNA. RAD51AP1 (RAD51 associated protein 1) expression was assessed using SABiosciences RT qPCR Primers, reference positions 991–1009, catalog number PPH08542A