Table 5.
Effect of RGC-32 knockdown on histone modifications and HDACs expression.
| Epigenetic mediators | shRGC-32/shCTR (normalised to actin) | shRGC-32/shCTR (normalised to total histone) |
|---|---|---|
| H2BK5ac | 3.5 | 2.9 |
| H3K9ac | 3.2 | 2.9 |
| H4K8ac | 2.4 | ND |
| H2BK15ac | 2.1 | 1.7 |
| H3K18ac | 1.7 | 2.7 |
| H2AK5ac | 1.0 | 0.9 |
| H3K9me2 | 0.8 | 0.9 |
| H3K27me3 | 0.6 | 0.8 |
| HDAC2 | 0.8 | NA |
| HDAC3 | 0.7 | NA |
| SIRT1 | 0.6 | NA |
The effect of RGC-32 knockdown on histone acetylation, methylation and HDACs expression was measured by western blot. The expression of these epigenetic mediators was quantitated by densitometric scanning and ratios to actin were calculated for both shRGC-32 and control-transfected cells. These ratios were used to calculate the RGC-32 shRNA to control shRNA-transfected cells ratio. NA = not applicable, ND= not determined