Fig. 4.
Manipulation of actin cytoskeleton affects VSMC differentiation in vitro. (A) latrunculin B (LatB, 0.5 µM for 6 hours) and Jasplakinolide (Jasp, 0.3 µM for 6 hours) induce significant morphology changes of PAC1 cells. (B–C) LatB and Jasp respectively reduce and enhance the transcription of VSMC-specific markers, SRF and myocardin by the real-time RT-PCR assay (B) and VSMC promoter activities (C) in PAC1 cells treated with LatB or Jasp for 6 hours. * and ** indicate p<0.05 and p<0.01 vs control.