Skip to main content
. Author manuscript; available in PMC: 2011 Feb 5.
Published in final edited form as: Mol Cell Endocrinol. 2009 Sep 17;315(1-2):246. doi: 10.1016/j.mce.2009.09.010

Fig. 3.

Fig. 3

Expression of Tcam1 mRNA and protein in the testis. In situ hybridization of Tcam1 as shown by darkfield (A) and lightfield (B) microscopy reveals that Tcam1 is expressed in male germ cells, mostly in pachytene spermatocytes. Expression of Tcam1 mRNA has a maximal point in the late pachytene spermatocytes of stage X tubules (D, black line). Immunofluorescence analysis of TCAM1 shows that it localizes along the germ cell membrane (C, inset) of mid-pachytene spermatocytes to early round spermatids (D, red line). Non-specific signals are a result of autofluorescence of spermatozoa and interstitial cells (data not shown).