Fig. 2.

(A) Confirmation of von Willebrand factor gene expression in EA.hy926 cells. Zero day means when the cells were at confluence. (B) Representative gene expression image of VCAM-1 and ICAM-1 of 6 hour zinc treatment in EA.hy 926 cells. The cells were exposed to TNFα (10 ng/ml) for 5 hours and after then, the cells were treated with TPEN (5 or 10 µM) and either Zn- (0 µM) or Zn+ (15 µM) for 6 hours. The experiment was done in triplicate.