Table 2.
Protein disulfide reductase activities of WhiD.
| Sample | Conc. (μM) | Reductase activity (× 10−3) (Ave. ΔA650 nm min−2) |
|---|---|---|
| Apo WhiD | 0.00 | 0.00 (±0.00) |
| 2.10 | 0.09 (±0.04) | |
| 6.26 | 0.41 (±0.06) | |
| 13.82 | 1.19 (±0.42) | |
| 15.64 | 1.62 (±0.12) | |
| 20.74 | 2.17(±0.35) | |
| Apo WhiDa | 3.15 | 0.30 (±0.06) |
| Holo WhiDb | 4.19 | 0.13 (±0.05) |
| Apo-FNR | 2.00 | 0.12 (±0.03) |
| ResA | 20.00 | 7.50c |
| Thioredoxin | 0.53 | 12.31 (±0.45) |
| 5.00 | 104.70 (±1.14) | |
a
Contained in vitro refolded apo-WhiD isolated from inclusion bodies
b
Conducted anaerobically.
c
Calculated from Lewin et. al. (71)