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Enumeration of tetramer+ CD4+ cells in PBMC from all vaccinates before and after immunization and peptide boost. Cryopreserved PBMC were stained with the tetramer and analyzed by FACS. PBMC were obtained 3 days before immunization (arrow) and at several time points thereafter and following the peptide boost on day 105 (arrow). Individual animals are indicated. The data represent the percentage of tetramer+ CD4+ cells of the total CD4+ T cells.
