Table I.
Primers used for constructing the DQA*1001/DQB*1002-F3-3 tetramer
| Construct | Name | Primer sequence (5′ to 3′) |
|---|---|---|
| QA*1001-blz-bs | DQA-bLZF | CTATGTCAGAGCTGACAGAGTCGTCAGCAGACCTGGTTCC |
| DQA-bLZR | GGAACCAGGTCTGCTGACGACTCTGTCAGCTCTGACATAG | |
| DQB*1002F3-3-alz-FLAG | DQB-aLZF | CTGAATCTGCCCAGAGCAAGTCGTCAGCAGACCTGGTTCC |
| DQB-aLZR | GGAACCAGGTCTGCTGACGACTTGCTCTGGGCAGATTCAG | |
| DQBRBR | TACACGAAATCCTTTGGGATCTCCCTGGCCCAG | |
| DRBQBF | CTGGGCCAGGGAGATCCCAAAGGATTTCGTGTA | |
| DQBLinkerF | TGGGTCCCCAAAGGATTTCGTGTA | |
| DQBLinkerR | TACACGAAATCCTTTGGGGACCCACCTCCTCCAGAGCCCCGTGG | |
| DQBF3-3R1 | TTTCTCTTGCCCCGCAGGGATCTCCCTGGCCCAGGC | |
| DQBF3-3R2 | TGCTCAGGTTCGGCAGTTTTCTCTTGCCCCGCAGG | |
| DQBF3-3R3 | AGTGAGCCACCACCTCCCGCAGCCTCATGCTCAGGTTCGGCAGT |