Table IV.
Reproducibility of tetramer staining
| Cell sourcea | Frequency (%) of tetramer+ CD4+ cells from animalb: | |
|---|---|---|
| 3892 | 3959 | |
| PBMC | ||
| Day 326 | 0.0078 +/− 0.0014 | NDc |
| Day 333 | 0.0063 +/− 0.0018 | 0.023+/− 0.0034 |
| Day 340 | 0.0029 +/− 0.0025 | ND |
| Day 348 | 0.0005 +/− 0.0005 | 0.0008+/− 0.0006 |
| Day 355 | ND | 0.0028+/− 0.0002 |
| Day 369 | 0.0008 +/− 0.0007 | 0.0004+/− 0.0003 |
| Day 384 | 0.0004 +/− 0.0003 | ND |
| Liver | 0.0019 +/− 0.008 | ND |
| Spleen | 0.0004 +/− 0.00005 | ND |
a
Cells were cryopreserved from PBMC or tissues at the indicated days post-immunization.
b
Results are presented as the mean frequency +/− 1SD of two to four replicate aliquots.
c
Not determined for more than one sample at these time points.