Figure 3.

Induction of Notch pathway genes by KSHV proteins. The 293T cells were transfected with expression vectors for latency phase genes (LANA or vFLIP; A) and lytic phase genes (RTA, vGPCR, or vIL-6; B). Empty pcDNA3 vector transfection was used as control. Expression of Notch receptors (left panel), Notch ligands (middle panel), and Notch pathway downstream genes (right panel) was analyzed by quantitative RT-PCR 72 hours after transfection. (C) The overall change of expression of Notch pathway genes after transfection was summarized. − indicates without significant induction; and +, with significant induction. (D) Expression of endothelial progenitor marker CD133 was analyzed by quantitative RT-PCR as in panels A and B.