FIG. 3.

Screening of respective whole-gel elution fractions that contain potential T-cell antigens. (A) Rotofor fraction 5 was fractionated into 14 subfractions (subfractions 1 to 14), and the protein bands were visualized by Coomassie blue staining. (B) Rotofor fractions 6 and 7 were subfractionated by whole-gel elution in a fashion identical to that described for Rotofor fraction 5. Subfractions of Rotofor fraction 5 (C) and subfraction 6.5 and 7.3 (D) were used to stimulate spleen cells from ΔiglC (KKF24)-primed or mock (PBS)-treated HLA-DR4 tg mice in an IFN-γ recall assay. Total TX-114-extracted proteins (TP) were used as a positive control for the cytokine recall assay. *, fractions selected for protein identification.