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FopB is a T-cell reactive antigen. CD4+ T cells were enriched from KKF24- or mock (PBS)-vaccinated HLA-DR4 tg mice and stimulated with increasing concentrations of rFopB in the presence of human APCs expressing HLA-DR4. The level of induction of IFN-γ was measured after stimulation for 72 h. Differences in IFN-γ stimulation between KKF24- and mock-vaccinated CD4+ T cells was significant at P values of <0.001 for rFopB (*) and <0.0001 for UV-inactivated KKF24 (**).
