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. 2009 Dec 2;17(2):239–246. doi: 10.1128/CVI.00421-09

FIG. 1.

FIG. 1.

Antibody isotypes induced by viral antigens. (A to C) WNV-specific IgG isotypes detected in sera of naturally infected alpacas by ELISA, using envelope (E) (A), nonstructural protein NS1 (B), and nonstructural protein NS5 (C). Horizontal bars represent mean OD values per group (n = 4). (D to F) WNV-specific IgG isotypes detected in sera of alpacas vaccinated three times (arrowheads) with killed, adjuvanted WNV. Antibodies specific for E protein (D), NS1 (E), or NS5 (F) were detected by ELISA in sera diluted 1:100. Bars represent standard deviations (SD) of the means (n = 8). Dashed, horizontal lines represent the limit of detection of the assay, calculated as the mean plus three times the average of the SD of the conjugate control.