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Ku70 and Ku80 protein levels in cytoplasm and nuclei of cancerous cell lines. 60 μg of nuclear and cytoplasmic proteins extracted from the cell lines were resolved by 10% PAGE. The Ku80, Ku70, AP-2α, AP-2γ and p185erbB2 proteins were revealed by immunoblotting. The antibodies are indicated on left. Beta-actin (β-actin) protein was used as a loading control.
