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. 2009 Nov 11;11(6):R83. doi: 10.1186/bcr2450

Figure 3.

Figure 3

Effect of Ku70/Ku80 and AP-2 downregulation on ERBB2 protein levels in BT-474 cells. (a) Immunoblot showing p185-erbB2, Ku70, Ku80, AP-2α, AP-2γ and beta-actin (β-actin) protein levels in cells transfected with the siRNAs indicated above the photograph. (b) Percent reduction of ERBB2 mRNA levels compared to the Neg siRNA condition in cells transfected with the different siRNAs indicated below the figure. ERBB2 mRNA levels were quantified in triplicate by Real-time RT-PCR, and corrected for beta-2-microglobulin mRNA levels.