Table 1.
Gene Name | Forward Primer (5'-3') | Reverse Primer (5'-3') | Product size (bp) | Primer location relative to TSS (bp) | Ref |
---|---|---|---|---|---|
p16INK4A-U | TTA TTA GAG GGT GGG GTG GAT TGT | CAA CCC CAA ACC ACA ACC ATA A | 151 | +132 to 283 | [22] |
p16INK4A-M | TTA TTA GAG GGT GGG GCG GAT CGC | GAC CCC GAA CCG CGA CCG TAA | 150 | +132 to 282 | |
HOXA9-U | TTG GGG TTA GAT AGG GAG TTG GGA | AAA AAT AAA AAC AAA AAA CAA ACA AA | 167 | -4453 to -4286 | [16] |
HOXA9-M | TCG GGG TTA GAT AGG GAG TCG GGA | AAA ATA AAA ACG AAA AAC AAA CGA A | 166 | -4453 to -4287 | |
CCND2-U | AGA GTA TGT GTT AGG GTT GAT T | ACA TCC TCA CCA ACC CTC CA | 108 | -1167 to -1059 | [12] |
CCND2-M | CGG CGA TTT TAT CGT AGT CG | CTC CAC GCT CGA TCC TTC G | 101 | -1138 to -1037 | |
RASSF1A-U | TTT GGT TGG AGT GTG TTA ATG TG | CAA ACC CCA CAA ACT AAA AAC AA | 108 | +201 to +309 | [23] |
RASSF1A-M | GTG TTA ACG CGT TGC GTA TC | AAC CCC GCG AAC TAA AAA CGA | 94 | +213 to +307 | |
SFRP1-U* | GTT TTG TAG TTT TTG GAG TTA GTG TTG TGT | CTC AAC CTA CAA TCA AAA ACA ACA CAA ACA | 135 | -2 to +133 | [24] |
SFRP1-M | TGT AGT TTT CGG AGT TAG TGT CGC GC | CCT ACG ATC GAA AAC GAC GCG AAC G | 126 | +2 to +128 | |
SFRP1-U** | TTT TAG TAA ATT GAA TTT GTT TGT GA | TAA AAT ACA CAA AAC TGG TAG AAC | 149 | -151 to -2 | [40] |
SFRP1-M | TTT AGT AAA TCG AAT TCG AAT TCG TTC GC | TAA AAT ACG CGA AAC TCC TAC G | 148 | -150 to -2 | |
p57-U | GTT GTT TGT GTT TGT GTA GTT TT | AAA AAT CCC ACA AAC AAC AAA ACA | 91 | +248 to +339 | |
p57-M | TTG TTC GCG TTT GCG TAG TTT C | AAA TCC CAC GAA CGA CAA AAC G | 88 | +249 to +337 | |
MGMT-U | TTT GTG TTT TGA TGT TTG TAG GTT TTT GT | AAC TCC ACA CTC TTC CAA AAA CAA AAC A | 93 | +30 to +123 | [25] |
MGMT-M | TTT CGA CGT TCG TAG GTT TTC GC | GCA CTC TTC CGA AAA CGA AAC G | 81 | +36 to +117 | |
THBS1-U | TTG AGT TTG TGT GGT GTA AGA GTA T | CCC CAC TAC CTA ACA CAC AAC T | 156 | -280 to -124 | [26] |
THBS1-M | GTT CGC GTG GCG TAA GAG TAC | CGC TAC CTA ACG CGC AAC T | 149 | -276 to -127 |
*exon 1
**promoter