FIG. 2.
The Shy1 complex is affected in CcO assembly mutants. Mitochondria were prepared from the wild-type (WT), cox11Δ, sco1Δ, and sdh2Δ strains with the genomically tagged versions of the respective proteins and analyzed by BN-PAGE as described for Fig. 1. (A to C) Distributions of Mss51-Myc (A), Coa1-Myc (B), and Shy1-Myc (C) complexes in cox11Δ, sco1Δ, and sdh2Δ mitochondria. (D) In vivo labeling of mitochondrial translation products. WT, sco1Δ, and sdh2Δ cells were pulsed for 15 min with [35S]methionine at 30°C. The reaction was stopped by addition of cold methionine, and samples were analyzed by 12% SDS-PAGE followed by autoradiography.